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Serum <em>Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis Article

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Engineering 2023, Volume 26, Issue 7,   Pages 151-158 doi: 10.1016/j.eng.2023.03.008

Abstract: Serum <em>Nem>-glycan profiles were tested using DNA sequencer-assisted fluorophore-assisted carbohydrateA total of nine <em>Nem>-glycan peaks were identified in serum samples for each subject.In distinguishing significant liver fibrosis, the diagnostic efficiency of the serum <em>Nem>-glycanIn distinguishing liver cirrhosis, the diagnostic AUROC curve of the serum <em>Nem>-glycan RF modelThe diagnostic efficiency of the constructed serum <em>Nem>-glycan models (RF-A and RF-B) was superior

Keywords： Liver fibrosis     Chronic hepatitis B     Serum <em>Nem>-glycan     <em>Nem>-glycan model     Alanine aminotransferase    

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Removable Dyes—The Missing Link for In-Depth <em>Nem>-Glycan Analysis via Multi-Method Approaches Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Engineering 2023, Volume 26, Issue 7,   Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

Abstract: To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effectiveThrough the detailed structural analysis of selected, dauntingly complex <em>Nem>-glycans from chickenovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategyMoreover, this approach "visualizes" <em>Nem>-glycans that have been difficult to identifyfar—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis     Mass spectrometry    

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Novel Insight into the Etiology of Haff Disease by Mapping the <em>Nem>-Glycome with Orthogonal Mass Article

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Engineering 2023, Volume 26, Issue 7,   Pages 63-73 doi: 10.1016/j.eng.2022.09.012

Abstract: Profiling of the <em>Nem>-glycome in HD would assist in deciphering the underlying molecular mechanismHerein, we enrolled 90 serum samples with HD patients and healthy controls from the Wuhan Center for<em>Nem>-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterizedobserved that HD is associated with an increase in the core fucosylation and mono-galactosylation of total serumThe serum level of IgG was found to serve as a good indicator for HD patients.

Keywords： Haff disease     Serum     Immunoglobulin G     Glycosylation     Disease pathogenesis    

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Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Frontiers of Medicine 2023, Volume 17, Issue 2,   Pages 240-262 doi: 10.1007/s11684-022-0936-z

Abstract: After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposedan EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent mannerRNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EMThe EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in pairedWithin individual gliomas, the EM/PM subtype was preserved across regions and single cells.

Keywords： glioma progression     molecular classification     EM/PM subtyping     intratumor heterogeneity    

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 6, doi: 10.1007/s11783-016-0884-4

Abstract: Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

Keywords： Flow cytometry     <em>Escherichia coliem>     <em>Staphylococcusaureusem>     UV     CTC     SYTO 9    

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Potential use of serum HBV RNA in antiviral therapy for chronic hepatitis B in the era of nucleos(t)ide

Fengmin Lu, Jie Wang, Xiangmei Chen, Dongping Xu, Ningshao Xia

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 502-508 doi: 10.1007/s11684-017-0590-z

Abstract: virus (HBV) are integrated into the life cycle of HBV replication, and the potential significance of serumThe production of HBV RNA virion-like particles should not be blocked by NA; in this regard, serum HBVTherefore, the concept of virological response is redefined as persistent loss of serum HBV DNA and HBVTherefore, the loss of serum HBV RNA would likely be a promising predicator for safe drug discontinuationThe clinical status of consistent loss of serum HBV RNA accompanied with low serum HBsAg levels might

Keywords： chronic hepatitis B     serum HBV RNA     nucleos(t)ide analogs     virological response     para-functional cure    

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Profound Diversity of the <em>Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Engineering 2023, Volume 26, Issue 7,   Pages 32-43 doi: 10.1016/j.eng.2022.08.016

Abstract: While the <em>Nem>-glycosylation of cell lines and serum has been widely examined, the analysis ofcancer-associated <em>Nem>-glycans from tissues has been hampered by the heterogeneity of tumors andthe complexity of <em>Nem>-glycan structures.<em>Nem>-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray>Nem>-glycans, as well as a decrease in bisected <em>Nem>-glycans.

Keywords： Colorectal cancer     Tumor     Porous graphitized carbon liquid chromatography mass spectrometry     N-glycomics     Antibody response    

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Effect of renal function and hemodialysis on the serum tumor markers in patients with chronic kidney

YU Xiaofang, XU Xialian, YE Zhibin

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 308-311 doi: 10.1007/s11684-007-0059-6

Abstract: with chronic renal failure, whether they have had hemodialysis or not, the specificity of some of the serumThe effects of renal function and hemodialysis were examined on serum levels of some of the tumor markersThere were no significant differences among the three groups in the serum concentrations of CA, AFP,of the corresponding tumors was decreased while serum AFP, CA, CA, PSA and fPSA were as valuable asHemodialysis further increased the serum level of CA and NSE.

Keywords： CKD     non-dialysis     valuable     detection     chronic    

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VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 4,   Pages 542-551 doi: 10.1007/s11705-016-1599-6

Abstract: We present the microbial green synthesis of silver nanoparticles (NPs) by VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30?50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

Keywords： <em>Streptomyces ghanaensisem> VITHM1     nanoparticles     3D structure     antibacterial activity    

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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Engineering doi: 10.1016/j.eng.2023.02.016

Abstract: To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effectivedetailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serumidentify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis     Mass spectrometry    

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Direct acting antiviral-induced dynamic reduction of serum

Tung Huynh, Ke-Qin Hu

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 658-666 doi: 10.1007/s11684-019-0707-7

Abstract: Direct acting antiviral (DAA) treatments may reduce the elevated α fetoprotein (AFP), but data on how these treatments affect elevated AFP in patients with chronic hepatitis C (CHC) remain insufficient. In the present study, the frequency of baseline AFP elevations and their related factors, AFP dynamics during and after DAA treatment, and factors associated with AFP reduction was assessed. This retrospective study included 141 patients with CHC without hepatocellular carcinoma who received DAA and achieved sustained virological response. The details are as follows: mean post-treatment follow-up was 99 weeks (12–213); mean age, 57.8 years old; 52%, males; 79%, genotype (GT) 1; and 47%, cirrhosis. Pre-treatment AFP elevation (>5.5 ng/mL) was seen in 48.2% patients. On multivariate analysis, baseline AFP>5.5 was associated with the presence of cirrhosis ( =0.001), co-existing non-alcoholic steatohepatitis (NASH) ( = 0.035), and GT 1 ( = 0.029). AFP normalization was seen in 28.2% patients at treatment week 2, in 52% at the end of treatment, and in 73.4% at the end of follow-up. Post-treatment week 24 AFP normalization was associated with the absence of cirrhosis ( = 0.003), Child–Pugh score<6 ( = 0.015), and baseline AFP<10 ( = 0.015). AFP elevation is common in patients with CHC and independently associated with NASH, cirrhosis, and GT 1. DAA treatment resulted in AFP normalization as early as treatment week 2. Post-treatment week 24 AFP normalization is independently associated with the absence of cirrhosis, Child–Pugh score<6, and baseline AFP<10.

Keywords： chronic hepatitis C     α fetoprotein     direct acting antiviral treatment     cirrhosis    

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Enhanced disinfection of <em>Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0854-x

Abstract: A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

Keywords： Photo-activated disinfection     Titanium dioxide     Nanowire membrane     Silver     Copper    

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein as a serum

Frontiers of Medicine 2022, Volume 16, Issue 3,   Pages 378-388 doi: 10.1007/s11684-021-0840-y

Abstract: Therefore, identifying serum biomarkers that indicate MPP progression and exploring new targeted drugsIn this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct

Keywords： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein     mechanistic target of rapamycin kinase inhibitor    

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Isolation and application of predatory

Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0900-3

Abstract: Indigenous predatory BALO strains were successfully isolated from activated sludge. Sludge SRF and CST were significantly reduced by BALOs induced biolysis process. The increase of BALO input dosage promoted the sludge biolysis efficiency. Sludge biolysis disintegrated flocs and lysed cells for internal water release. The optimal sludge biolysis time was 24 h and no pH adjustment was needed. -and-like organisms (BALOs) are a group of ubiquitous and obligate predatory bacteria and commonly used as biocontrol agents. In this study, an efficient, environmental-friendly, and convenient BALOs encouraged municipal waste sludge biolysis pretreatment technique was developed and investigated for dewaterability enhancement of excess waste sludge. The indigenous predatory BALOs were successfully isolated from the sludge for biolysis treatment. Without any chemical addition or pH adjustment, the sludge specific resistance (SRF) and capillary suction time (CST) were significantly reduced by as high as 53.4% and 23.8%, respectively within 24 h’s treatment, which would further be lowered with the increase of BALOs input dosage. However, the continuous extension of reaction time would worsen the sludge dewaterability. The decreases of SRF and CST accompanied with the increases of sludge disintegration degree and soluble chemical oxygen demand, nitrogen, and phosphorus concentrations all emphasized the contributions of BALOs’ predation activities to sludge disturbance, cell lysis, and consequently the release of sludge intracellular water to finally effectively improve the sludge dewaterability and disposal efficiency.

Keywords： <em>Bdellovibrioem>-and-like organisms (BALOs)     Biolysis     Activated sludge     Dewaterability     Predation    

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High-Throughput Profiling of Serological Immunoglobulin G <em>Nem>-Glycome as a Noninvasive Biomarker Article

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Engineering 2023, Volume 26, Issue 7,   Pages 44-53 doi: 10.1016/j.eng.2023.02.008

Abstract: >Nem>-glycome.A Lasso algorithm, accompanied by a logistic regression model, was used to develop a glycan-based modelIn addition, the composition of IgG <em>Nem>-glycans was correlated with that of inflammatory cytokines(<em>rem> = 0.556).The glycan-based models for diagnosing GI cancers exhibited an excellent performance, with areas under

Keywords： Gastrointestinal cancer     Glycosylation     Immunoglobulin G     Diagnostic biomarker    

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Title Author Date Type Operation

Serum <em>Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Journal Article

Removable Dyes—The Missing Link for In-Depth <em>Nem>-Glycan Analysis via Multi-Method Approaches

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Journal Article

Novel Insight into the Etiology of Haff Disease by Mapping the <em>Nem>-Glycome with Orthogonal Mass

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Journal Article

Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Journal Article

Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Journal Article

Potential use of serum HBV RNA in antiviral therapy for chronic hepatitis B in the era of nucleos(t)ide

Fengmin Lu, Jie Wang, Xiangmei Chen, Dongping Xu, Ningshao Xia

Journal Article

Profound Diversity of the <em>Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Journal Article

Effect of renal function and hemodialysis on the serum tumor markers in patients with chronic kidney

YU Xiaofang, XU Xialian, YE Zhibin

Journal Article

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Journal Article

Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

Journal Article

Direct acting antiviral-induced dynamic reduction of serum

Tung Huynh, Ke-Qin Hu

Journal Article

Enhanced disinfection of <em>Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Journal Article

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein as a serum

Journal Article

Isolation and application of predatory

Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li

Journal Article

High-Throughput Profiling of Serological Immunoglobulin G <em>Nem>-Glycome as a Noninvasive Biomarker

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Journal Article